Laboratories are equipped with all kinds of instruments and measuring tools. They are used for pharmaceutical, chemical, or medical examinations and analyses, tests, and quality controls. Many applications require oil-free compressed air and N2 gases of high purity. Oil vapors can damage valves, clog nozzles, contaminate the air, or falsify measurement results.
Our products produce the purest Nitrogen gases to meet the requirements and enable analytical procedures and the ability to carry out work at the highest level.
1. High-Purity Nitrogen and its Application in Mass Spectrometry
In contemporary laboratory settings, the majority are outfitted with a comprehensive array of specialized equipment and measurement instrumentation tailored for multifaceted applications. These facilities play a critical role in conducting pharmaceutical, chemical, and medical analyses, as well as rigorous testing and quality assurance protocols. Numerous procedures mandate the utilization of oil-free compressed air and gases characterized by stringent quality and purity criteria. The presence of oil vapors introduces potential deleterious effects, including valve degradation, nozzle obstruction, air contamination, and potential aberrations in measurement outcomes.
The utilization of dry and high-purity compressed air is imperative for laboratory instruments. Our products offer nitrogen gas with unparalleled purity; adhering to rigorous specifications, thereby facilitating sophisticated analytical methodologies and ensuring optimal operational performance.
2. Liquid Chromatography-Mass Spectrometry (LC-MS)
Liquid chromatography-mass spectrometry (LC-MS) is a fundamental technique in analytical chemistry, pivotal in the pharmaceutical and toxicology fields. It excels in separating and identifying complex compounds. In pharmaceuticals, LC-MS aids in drug discovery, detecting metabolites and degradation products. In toxicology, it identifies xenobiotics and their metabolites in biological samples. Additionally, LC-MS offers insights into metabolic pathways, enhancing drug safety and efficacy evaluations.
How does it work?
LC-MS combines liquid chromatography’s separation with mass spectrometry’s ionization. After chromatographic separation, compounds are ionized in the mass spectrometer, and the resulting ions are sorted by mass-to-charge ratios for precise identification and quantification.
Nitrogen application in LC-MS
In LC-MS, nitrogen serves as a vital component for several critical functions. It provides a clean and inert environment, aiding in the ionization process by displacing unwanted gases. Additionally, nitrogen acts as a nebulizing gas in certain ionization techniques, ensuring efficient sample introduction. Its high purity ensures minimal interference with analytical results, facilitating accurate compound identification and quantification.
3. Nitrogen in Thermogravimetric Analysis
Thermogravimetry (TG) is a thermal analysis technique that measures the weight change of a sample as a function of temperature or time in a controlled atmosphere. As the sample undergoes heating, its mass loss or gain is recorded, providing insights into processes such as decomposition, oxidation, and phase transitions. This method is particularly valuable for determining the thermal stability, composition, and kinetic parameters of materials, offering crucial information for various scientific and industrial applications.
In thermogravimetry, nitrogen serves as an essential inert atmosphere. It prevents sample oxidation and ensures accurate weight measurements by eliminating potential reactions with oxygen, thereby preserving the sample’s integrity during thermal analysis.
4. Nitrogen for Multigas Incubators (Tri-Gas)
Multigas incubators, often referred to as tri-gas incubators, function by controlling the internal atmosphere to mimic specific physiological conditions for cell culture or microbial growth. Typically, these incubators regulate the levels of oxygen, carbon dioxide, and nitrogen to simulate in vivo environments. This precise gas control ensures optimal cell viability, growth, and functionality by providing conditions tailored to the specific requirements of the cultured organisms or cells.
In tri-gas incubators, nitrogen stabilizes the atmosphere, balancing oxygen and carbon dioxide levels essential for specific cell or microbial cultures. It mitigates oxidative stress, ensuring optimal growth and viability of the organisms or cells.
5. Nitrogen for sample preparation/solvent evaporator
In sample preparation, solvent evaporation is pivotal for concentrating solutions prior to analysis. Utilizing methods like rotary evaporation or nitrogen blow-down, solvents are efficiently removed while maintaining the sample’s integrity. Precise control of evaporation parameters is crucial for reproducible and accurate analytical outcomes in subsequent assays.
In sample preparation, the use of nitrogen in evaporators serves a dual purpose. Nitrogen acts as a non-reactive gas to displace oxygen, reducing the risk of sample oxidation during the evaporation process. Additionally, by creating an inert atmosphere, nitrogen facilitates the efficient and rapid evaporation of solvents, aiding in the concentration of samples without compromising the integrity of sensitive analytes. This controlled environment ensures optimal conditions for sample preparation, enhancing the reliability and accuracy of subsequent analyses.
